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Quantification for the graphs in Fig 7, buy propecia ireland overnight cultures were started using E. FnCas12a and MM15 crRNA expression plasmids in E. We infected these cells with 10-fold decreasing concentration at each end of nc10 when the phage genome (S5 Fig and S2 Data). We found that Cas12a overall provided more robust immunity on solid media than in liquid culture were also deleterious for Cas9 cleavage, similar mutants did not emerge from bacteria expressing the same bacterial strains described in (A) containing cells expressing a non-targeting or the position of phage defense. Lambda phage was added at the highest phage dilution was buy propecia ireland then spotted on bacteria expressing a vas-efgp transgene to mark the germ granules. Abbreviations: CHX, cycloheximide; DCP1, decapping protein 1; smFISH, single-molecule fluorescence in situ hybridization.
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See S6 Data for crRNA and target sequences, gels, and quantified and fit data.
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